MicroManagerTiffImagingExtractor#

class MicroManagerTiffImagingExtractor(folder_path: str | Path)[source]#

Bases: MultiImagingExtractor

Specialized extractor for reading TIFF files produced via Micro-Manager.

The image file stacks are saved into multipage TIF files in OME-TIFF format (.ome.tif files), each of which are up to around 4GB in size. The ‘DisplaySettings’ JSON file contains the properties of Micro-Manager.

Create a MicroManagerTiffImagingExtractor instance from a folder path that contains the image files.

Parameters:

folder_path (PathType) – The folder path that contains the multipage OME-TIF image files (.ome.tif files) and the ‘DisplaySettings’ JSON file.

get_image_shape() tuple[int, int][source]#

Get the shape of the video frame (num_rows, num_columns).

Returns:

image_shape – Shape of the video frame (num_rows, num_columns).

Return type:

tuple

get_sampling_frequency() float[source]#

Get the sampling frequency in Hz.

Returns:

sampling_frequency – Sampling frequency in Hz.

Return type:

float

get_num_samples() int[source]#

Get the number of samples in the video.

Returns:

num_samples – Number of samples in the video.

Return type:

int

get_dtype() dtype[source]#

Get the data type of the video.

Returns:

dtype – Data type of the video.

Return type:

dtype